Literature summary for 3.4.21.72 extracted from

Kotelnikova, O.V.; Zinchenko, A.A.; Vikhrov, A.A.; Alliluev, A.P.; Serova, O.V.; Gordeeva, E.A.; Zhigis, L.S.; Zueva, V.S.; Razgulyaeva, O.A.; Melikhova, T.D.; Nokel, E.A.; Drozhzhina, E.Y.; Rumsh, L.D.
Serological analysis of immunogenic properties of recombinant meningococcus IgA1 protease-based proteins (2016), Bull. Exp. Biol. Med., 161, 391-394 .

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme variants in Escherichia coli inclusion bodies	Neisseria meningitidis serogroup B

Protein Variants

Protein Variants	Comment	Organism
additional information	creation of four producer strains with effective expression of recombinant proteins (in the form of inclusion bodies) that represent variants of IgA1pr from Neisseria meningitidis serogroup B (strain B44/76) with different primary structures carrying C-terminal histidine label (-LEH6). Protein I is enzymatically active IgA1pr containing M1K2-N963 sequence. Protein IIa (MA28-N963) also possessing specific enzymatic activity is a variant of protein I lacking signal peptide (M1K2-A27). Enzymatically inactive protein IIm is a variant of protein IIa with catalytically active Ser267 residue substituted with an Ala residue. Protein III, a low-molecular-weight fragment of IgA1pr (ME135-H328), is enzymatically inactive and contains a sequence in the IgA1pr region with high density of potential T- and B-cell epitopes	Neisseria meningitidis serogroup B

Organism

Organism	UniProt	Comment	Textmining
Neisseria meningitidis serogroup B	-	-	-
Neisseria meningitidis serogroup B B44/76	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme variants solubilized from Escherichia coli inclusion bodies by nickel affinity chromatography, dialysis, and anion exchange chromatography	Neisseria meningitidis serogroup B

Synonyms

Synonyms	Comment	Organism
IgA1 protease	-	Neisseria meningitidis serogroup B
IgA1pr	-	Neisseria meningitidis serogroup B

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Release 2023.1 (January 2023)